Plasmid:

LentiCRISPRv2-SFFV-KRAB-dCas9: A single lentiviral vector plasmid that expresses both the guide RNA and KRAB-dCas9 fusion protein to repress any gene by CRISPRi.

Ref: Biswas et al., Cancer Discovery, 2022.

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Cell lines:

Metastatic cell line that induces cachexia: C26m2 (colon cancer cell line)

Ref: Wang, Biswas, Ma et al., Nat Med, 2018.

 

Brain metastatic cell line- H1650-BrM.

Ref: Biswas et al., Cancer Discovery, 2022.

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Antibodies:

Anti-human and anti-mouse ZIP14 antibodies.

Ref: Wang, Biswas, Ma et al., Nat Med, 2018.